Alignment Quests will appear once you hit 25% in a particular alignment. Currently there is no quest for Truthful.
It is possible to get quests from opposing alignments as long as you reach 25% in both directions of this alignment.
|Peril of the Old Ways||Blood's New Ways|
|Threat to the Realm||Treason in the Family|
NOTE: Alignment quests are not activated as soon as you hit 25% of a certain alignment.
Based on my personal observation, the pre-requisite quest for them is Abduction in the Woods quest of the Family Reckoning storyline. At the time of this entry I am playing as Martell, and upon finishing this quest, 2 alignment quests were activated at the same time, one with 28% and the other with 30% alignment.
Please post your own observations in the topic I started on our forum.